Determination of sulfonamides residues in animal muscle by HPLC means

The method of High Performance Liquid Chromatography (HPLC) was used to determinate the sulfonamides(SMM? SDM? SM2? SMZ? SQ) residues in seven kinds of animal muscle. The drugs were extracted with acetonitrile, and the fat was removed by extracting into hexane, then the extracts were evaporated. The residues were dissolved and pured by Alumina B cartridges SPE clumn, the recovery rate of detected sample was 60%～110%. The lowest limit of detection was 2 μg/kg, the lowest limit of quantification was 10 μg/kg, and the inte-assay variation was less than 15%, the intra-assay variation was less than 20%.