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Determination of palmatine and jatrorrhizine in Tinospora segittata and T.sagittata Var Cravernina by RP-HPLC

Tianzhi Wang

Open publisher page 1 citations

Abstract

OBJECTIVE To establish an HPLC method to determine the contents of palmatine and jatrorrhizine in Tinospora segittata and T.sagittata Var Cravernina.METHODS The HPLC condition was ODS column,acetonitrile-water(1∶1,containing 3.4 g KH 2PO 4 and 1.7 g CH 3(CH 2) 11OSO 3Na per 1000 ml) as mobile phase and 345 nm as detective wavelength.RESULTS The average recoveries were 97.64% and 98.77% for palmatine and jatrorrhizine, respectively.CONCLUSION The method is accurate,simple and sensitive.It can be used for the quality control of Jinguolan.

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OBJECTIVE To establish an HPLC method to determine the contents of palmatine and jatrorrhizine in Tinospora segittata and T.sagittata Var Cravernina.METHODS The HPLC condition was ODS column,acetonitrile-water(1∶1,containing 3.4 g KH 2PO 4 and 1.7 g CH 3(CH 2) 11OSO 3Na per 1000 ml) as mobile phase and 345 nm as detective wavelength.RESULTS The average recoveries were 97.64% and 98.77% for palmatine and jatrorrhizine, respectively.CONCLUSION The method is accurate,simple and sensitive.It can be used for the quality control of Jinguolan.

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Available abstract

OBJECTIVE To establish an HPLC method to determine the contents of palmatine and jatrorrhizine in Tinospora segittata and T.sagittata Var Cravernina.METHODS The HPLC condition was ODS column,acetonitrile-water(1∶1,containing 3.4 g KH 2PO 4 and 1.7 g CH 3(CH 2) 11OSO 3Na per 1000 ml) as mobile phase and 345 nm as detective wavelength.RESULTS The average recoveries were 97.64% and 98.77% for palmatine and jatrorrhizine, respectively.CONCLUSION The method is accurate,simple and sensitive.It can be used for the quality control of Jinguolan.

Key concepts: Jatrorrhizine, Palmatine, Chemistry, Chromatography, High-performance liquid chromatography, Traditional medicine, Medicine

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Determination of palmatine and jatrorrhizine in Tinospora segittata and T.sagittata Var Cravernina by RP-HPLC — Research Paper | ScholarLens