2008•Journal of Instrumental AnalysisRequires access

Simultaneous Determination of Jatrorrhizine,Palmatine and Berberine in Mahonia duclouxiana Gagnep.by HPLC

Buming Liu

Abstract

A high-performance liquid chromatography(HPLC) method has been developed for simultaneous determination of jatrorrhizine,palmatine and berberine in Mahonia duclouxiana Gagnep..The sample was treated by ultrasonic extraction with HCl-MeOH solution(1∶100,by volume).The filtrate was separated on a C18 column by using acetonitrile-0.05 mol/L KH2PO4(28∶72,by volume) solution,whose pH was adjusted to 3.0 with H3PO4,as mobile phase,and detected at 265 nm.The average recoveries of jatrorrhizine hydrochloride,palmatine hydrochloride and berberine hydrochloride from spiked sample were 99%,100% and 99%,respectively.The relative standard deviations were 1.43%,1.93% and 2.82%(n=9),respectively.

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Key concepts: Jatrorrhizine, Palmatine, Chemistry, Chromatography, Berberine, Berberine hydrochloride, High-performance liquid chromatography, Acetonitrile