Determination of Palmatine and Jatrorrhizine in Tinospora capillipes Gagn.By Non-aqueous Capillary Electrophoresis

OBJECTIVE To establish a non-aqueous capillary electrophoresis method for the separation and determination of palmatine and jatrorrhizine in Tinospora capillipes Gagn.from different original area.METHODS An uncoated fused-silica capillary(50.0 cm×75 μm) was used for separation in this study.The mobile phase consisted of 75 mmol·L-1 ammonium acetate-0.1% acetic acid in methanol.The applied voltage was 15 kV,and the column temperature was maintained at 25 ℃.The chromatogram was detected at 265 nm.RESULTS The linearities were obtained over the range of 1.99~99.5 mg·L-1 for palmatine,and the range of 2.26~113 mg·L-1 for jutrorrhizine.The average recoveries were 101.9% for palmatine,and 100.9% for jatrorrhizine,respectively.CONCLUSION This method is simple,rapid and reproducible,and it is successfully employed for the determination of palmatine and jatrorrhizine in T.capillipes Gagn.and other Chinese herbs containing these alkaloids.