GP Gene Sequencing of Three Passages of Current Rabies Vaccine Strain CTN-1-V in China

Objective To analyze the sequence of glycoprotein(GP) gene of three passages of rabies vaccine strain CTN-1-V in China and compare with that of street virus strain.Methods The GP cDNA fragments were amplified from the murine brain infected with CTN-1-V strain by RT-PCR then sequenced.Results The homologies of nucleic acid and amino acid sequences of three passages of CTN-1-V strain to those of street strain were 83.2%-96.8% and 90.0%-97.4% respectively.The nucleic acid sequences of three passages of CTN-1-V strains were basically identical.Conclusion The structure of GP gene of CTN-1-V strain was bascially stable during passage,and the homology of CTN-1-V to street strain was higher than those of aG and PV strains.