Clinical application of time-resolved immunofluoresce for detection of hepatitis b virus pre S1 antigen

Objective:Using time-resolved immunofluorescence assay(TRFIA) to detect hepatitis B patients pre-S1 antigen and comparing the results of enzyme-linked immunosorbent assay(ELISA) and HBV-DNA measured results.Methods:TRFIA method and ELISA method were used separately to detect serum pre-S1 antigen of 362 cases of hepatitis B patients.HBV-DNA was detected to the specimens which PreS1 antigen results did not meet by two methods and some pre-S1 antigen test results are near Cutoff.Results:Using the ELISA method as a reference,the positive coincidence rate of TRFIA method was 98.8% and the negative coincidence rate was 75.7%.The overall coincidence rate was 92.0% and the difference between the two methods had statistical significance.The positive rate of TRFIA method was higher than ELISA method.Comprised With hepatitis B virus DNA results.The TRFIA method had no significant difference.And the ELISA method had statistical significance.Conclusion:Compared with the ELISA method,detection of pre S1 antigen with TRFIA method can better reflect the hepatitis B virus replication.