Optimization of fermentation condition of producing lipase by Candida valida

Candida valida was used to produce lipase.The optimization of fermentation condition about culture medium composition and culture condition of extracellular lipase produced was as follows:polyethanolen olive oil and glucose as carbon source,peptone and urea as nitrogen source,yeast extract 4.5g/L,MgSO4.7H2O 0.4g/L,K2HPO4 3.5g/L;culture temperature 30℃,initial pH 7.0,inoculation volume 10%,speed of agitation 180r/min culturing 24h.Under these conditions,the maximum lipase activity of the Candida valida can reach up to 12U/mL.