Fermentation process optimization and enzymology properties research for lipase production by Candida Antarctica

The nutrient medium composition and operation conditions for producing extracellular lipase from Candida Antarctica are studied in both shake-flask and 15 L fermenter. The optimal nutrient medium contains soybean meal 40 g/L, starch 15 g/L, soybean oil 5 mL/L, K2HPO4 4 g/L, MgSO4 .7H2() 1 g/L, Tween-80 0. 1%, yeast extract 5 g/L. The optimal operation conditions are as follows; temperature 24 C , initial pH 6. 0, airflow rate 10. 0 L/min. Under the optimal conditions, the period of fermentation reduces to 54 h. The maximum lipase activity of 19. 6 U/mL is achieved in 15 L fermenter. The lipase is stable in pH ranges of 4. 0-6. 0 and 7. 5-9. 0 and has an optimal activity at pH range of 6-8. 5. The lipase has the highest activity at 70℃ and is stable in range of 40-70℃ with more than 60% activity remaining after exposure of 1 h.