Optimization of conditions for producing lipase by candida valida

The culture medium ingredient and the optimal raising condition of producing extracellular lipase by candida valida were studied.The results indicated that the best culture medium composition was:10 g/L glucose +20 g/L olive oil,6 g/L yeast paste +4 g/L protein peptone,K2HPO4:2 g/L,MgSO4:2 g/L,and the optimal culture condition was the initial pH 7.5,the raising temperature 30 ℃,the installing liquid volume was 30mL(250mL triangle bottle),the inoculating quantity was the 1%,180 rad/min raising 48 h,the nutrient fluid enzyme reached to 16.36 U/mL.