Effects of GLP-II pretreatment on mouse intestinal mucosal immunity after ischemia/reperfusion insult

Objective: To explore the protections of GLP-Ⅱ pretreatment on mouse intestinal mucosal immunity after ischemia/reperfusion insult. Methods: Thirty ICR mice were randomly divided into three groups: namely normal control (N), control (C) and GLP-Ⅱ pretreatment (P). Group C and group P were inflicted with ligation of superior mesenteric artery for twenty minutes. The morphology of distal ileum mucosa, the rate of intestinal bacteria translocation, the level of plasma endotoxin and intestinal IgA were determined. Results: After ischemia/reperfusion insult, there were obvious damage at distal ileum mucosa in group C and obvious proliferation in group P.The rate of intestinal bacteria translocation and the level of plasma endotoxin were significantly increased (P0.01), and the levels of intestinal IgA were significantly decreased (P0.01) in groups C and P. Comparing with group C, the rate of intestinal bacteria translocation and the level of plasma endotoxin were lower (P=0.005) and the levels of intestinal IgA were higher (P0.01) in group P. Conclusion: The intestinal mucosal barrier may be severely damaged after ischemia/reperfusion insult. The preteatment of GLP-Ⅱ can relieve the damage of intestinal mucosal barrier.