Study on enzymatic hydrolysis of chicken bone protein

Different kinds of proteases were used to hydrolyze chicken bone protein to make hydrolyzed animal protein(HAP).Results showed that the degree of hydrolysis(DH) was higher after being hydrolyzed by pancreatin,AS.1398 neutral protease,or Alcalase than the other proteases.The optimum hydrolysis conditions of pancreactin were: digestion at pH 8.5 and 50 ℃ for approximately 60 min,at an enzyme-substrate ratio of 0.75∶100.And the optimum conditions of AS.1398 neutral protease were: digestion at pH 7.5 and 55 ℃ for 60 min,at an enzyme-substrate ratio of 3∶100.While for Alcalase,the optimum conditions were at the temperature of 60 ℃ at pH 8.5 for 60 min,at an enzyme-substrate ratio of 1∶100.