2008•Chinese Journal of Public HealthRequires access
Effects of bFGF on the expression of CaMK II mRNA in hippocampus and cortex after cerebral ischemia and reperfusion in rats
Xing Xue-song
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Abstract
Objective To investigate the expression of CaMK Ⅱ mRNA in hippocampus and cortex after cerebral ischemia reperfusion in rats,explore the regulative effects and the mechanism of basic fibroblast growth factor(bFGF) on CaMK Ⅱ mRNA in the brain tissue of the rats.Methods The model of middle cerebral artery occlusion(MCAO) was performed by using the intraluminal filament method.The expression of CaMKⅡ mRNA and neuron apoptosis in hippocampus and cortex were detected with RT-PCR TUNEL method.Results The expression of CaMK Ⅱ mRNA in hippocampus and cortex reduced after cerebral ischemia reperfusion.The expression of CaMK Ⅱ mRNA in bFGF group was increased,but neuron apoptosis decreased.Conclusion Basic fibroblast growth factor neuron inhibits apoptosis and participates in the regulation of expression of CaMK Ⅱ mRNA in ischemic neurons and protects the neurons.