Effects of wilforine on the secretion of inflammatory factors from lipopolysaccharide-induced RAW264.7 cells

OBJECTIVE To investigate the effects of wilforine on the release of inflammatory cytokines from lipopolysaccharide-induced RAW264.7 cells.METHODS The inflammation model was constructed by treating RAW264.7 cells with lipopolysaccharide(LPS).The cytotoxicity of wilforine with different doses was examined by cell counting kit-8(CCK-8)method;the levels of TNF-α,IL-6 were tested by ELISA and NO was assayed by nitrate acid reductase method.RESULTS Wilforine showed no cytotoxicity on the growth of RAW264.7 cells at the concentrations from11.5 to 115μmol·L-1(P0.05);LPS induced markedly increased secretions of TNF-α,IL-6 and NO from RAW264.7 cells;Meanwhile,wilforine significantly decreased the levels of TNF-α,IL-6 and NO induced by LPS(P0.05,P0.01).CONCLUSION Wilforine has anti-inflammatory effect,which might be mediated by down-regulation of the expression of inflammatory factors TNF-α,IL-6 and NO.