Inhibitory effects of parthenolide on cytokine expression in RAW264.7 cells

Aim: To study the inhibitory effects of parthenolide on LPS induced cytokine expression in RAW264. 7 cells. Methods: Quantitative reverse transcription real-time polymerase chain reaction and enzyme linked immunosorbent assay were used to determine some cytokines' expression at mRNA levels( IL-6,TNF-α,Ptgs2,IL-1β,IL-10,MMP13 and CXCL1) and at protein levels( IL-6,TNF-α,IL-1βand IL-10) after treatment of parthenolide( 0. 1,1,10,50 μmol / L) and Lipopolysaccharide( LPS,10μmol / L) in RAW264. 7 cells. Four control groups were included in the study. They were DMSO group,LPS group,DMSO + LPS group,and Bay 11-7082 + LPS group. Results: The mRNA expression levels of IL-6,TNF-α,Ptgs2,IL-1β,IL-10 and MMP13 were significantly decreased by 10 or 50 μmol / L parthenolide after RAW264. 7 cells were treated with 10 μmol / L LPS for 8 hours. However,no significant change of CXCL1 in mRNA expression level was observed. The protein levels of IL-6,TNF-α,IL-1β and IL-10 were also found to be inhibited by 10 or 50 μmol / L parthenolide after RAW264. 7 cells were treated with 10 μmol / L LPS for 24 hours. Conclusion: Cytokines' expression induced by LPS in RAW264. 7cells was arrested by parthenolide.