2007•Anhui nongye kexueRequires access
Detection of β-agonist in Feedstuff by LC/MS
Caiyun Liu
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Abstract
Detection of clenbuterol and ractopamine in feedstuff was studied by liquid chromatography mass spectrometry(LC/MS).The clenbuterol and ractopamine of specimen was extracted by 0.5 % tungstenic acid: methanol(80∶20).It was then purified by Oasis MCX.The mobile phase was 4 mmol/L ammonium acetate∶acetonitrile(80∶20).PDA detector was used for separation and detection.Detection limits of clenbuterol and ractopamine were 1.05 μg/kg and 1.37 μg/kg and the average rates of their recovery were 89.7 % and 84.4 %,respectively.The method was simple and precise,which could be used for the detection of clenbuterol and ractopamine in feedstuff.