Effect of salvia ligustrazine injection on the efficacy of hypertension pregnancy model ratsand the expression of placental cell Cyclin B1 and p21 protein

Objective To investigate the effects of salvia injection on the effect of TMP rat model of hypertension in pregnancy and placental cells CyclinB1,p21 protein expression. Methods 64 rats during pregnancy were divided into four groups: normal pregnant rats as control,pregnancy-induced hypertension rats as negative control,pregnancy-induced hypertension rats treated with magnesium sulfate as positive control,pregnancy-induced hypertension rats treated with salvia ligustrazine injection as experimental group,each had 12 rats. Control group and negative group rats were given the same dose of the drug saline; positive group and the experimental group were separately given magnesium sulfate and salvia ligustrazine injection therapy,three days a dosing regimen. Blood pressure in rats tails were measured,24 h urine protein though tail vein blood were determined,newborn rats weight,still birth rate,limb deformity rate and placental weight of pregnant rats in each group were observed,and placental cell CyclinB1,p21 protein expression were detected by Western blott. Results Compared with control group,blood pressure and urine protein of pregnant rats in negative control group,were increased significantly( P 0. 05),neonatal height,weight and placental weight were decreased significantly( P 0. 05),and the stillbirth rate and malformation rate was significantly higher( P 0. 05). Compared with negative control group,blood pressure and urine protein of pregnant rats in positive control group and experimental group were decreased significantly( P 0. 05),neonatal height,weight and placental weight were increased significantly( P 0. 05) and the stillbirth rate and malformation rate was significantly lower( P 0. 05). There were no significant differences of those indexes between positive control group and experimental group. Compared with control group,cyclinB1 protein expression in negative control group was decreased( P 0. 05),and p21 was significantly increased( P 0. 05),Compared with negative control group,cyclinB1 protein expression in positive control group and experimental group were all increased( P 0. 05),ang p21 was significantly decreased( P 0. 05). There were no significant differences of those indexes between positive control group and experimental group. Conclusion TMP injection can significantly reduce pregnancyinduced hypertension in pregnant rats and deformity rate,stillbirth rate,neonatal rats situation also improved,and can increase cyclinB1 expression and decrease p21 expression,which may be one of the therapeutic effect of TMP injection reasons.