Influence of transection of the cervical sympathetic trunk on content of NO and expression of NOS in placenta of rats with pregnancy-induced hypertension syndrome

AIM:To observe the influence of transection of the cervical sympathetic track(TCST)on the content of NO and the expression of eNOS mRNA and iNOS mRNA in placenta of the rats with pregnancy-induced hypertension syndrome(PIH).METHODS:Pregnant Wistar rats were randomly divided into 5 groups:control group(C):saline was injected subcutaneously from 14th day to 20th day of gestation;PIH group 1(H1)and group 2(H2):L-NAME was respectively injected with 125 mg/kg and 62.5 mg/kg,respectively,then the other procedures were the same as group C;Operation group(O):TCST was operated on 14th day of the gestation,then the other procedures were the same as group H1;sham operation group(S):the cervical sympathetic trunk was only separated and exposed on 14th day of the gestation,then the other procedures were the same as group H1.RESULTS:(1)Except the base value of the BP and protein in urine of the pregnant rats,all the parameters observed in group H1 and H2 were higher than those in group C significantly(P0.01),and in group H2 were lower than those in group B1 markedly(P0.01).(2)In comparison with those in group C,the size and body weigh of fetus in group H1,H2 decreased markedly(P0.01).The above indexes in group H1 were lower than those in group H2 markedly(P0.01,P0.05).The changes of the rate of embryo absorption and fetal death,and deformity rate of the fetal rats were contrary to the above indexes.(3)The content of NO and the expression of eNOS mRNA and iNOS mRNA in placenta in group H1 and H2 were lower than those in group C markedly(P0.01).Those in group H1 were lower than those in group H2 obviously(P0.01,P0.05).Those in group O were higher than those in group H1 markedly(P0.01).CONCLUSION:TCST protects pregnant rats against PIH,and it was related to the mRNA expression of eNOS and iNOS and the content of NO in placenta tissue.