Effect of Carbon and Nitrogen Sources on Lignin Degradation Ability and Enzyme Activity of Dual-Cultured,Lignin-Degrading Fungi

White-rot fungi are characterized by their lignin-degrading capability derived from their ligninolytic enzyme system.Carbon and nitrogen sources are important factors for the lignin degradation rate and enzyme activity of these fungi.The present study was conducted to investigate the effect of selected carbon and nitrogen sources on lignin degradation ability,ligninolytic enzyme activity and crude protein con-tents by dual culture of two Pleurotus strains(P.pulmonarius Tf1 and P.cornucopiae JG1)on wheat straw substrates.Lignin peroxidase(Lip) and manganese-dependent peroxidase(MnP)were the major lignin-degrading enzymes for the dual-cultured fungi Tf1+JG1.Carbon source(glucose,cane sugar,maizena,maltose and starch)and nitrogen source(ammonium tartrate,ammonium sulfate,peptone,wheat bran,soybean meal)were tested in these experiments.The greatest lignin degradation ability of Tf1+JG1 was found using glucose as carbon source and am-monium tartrate as a nitrogen source.After fermentation for 9 days,weight loss for wheat straw was 14.87%,lignin content was 8.68% and lignin degradation was 22.95%.The crude protein content was 7.28%,which is 36.84% greater than non-fermented wheat straw.The Lip and MnP activities were 629.11 U.g-1and 622.22 U.g-1,respectively.