Genetic Diversity Analysis of Potato Germplasm by SRAP Makers
Jichun Wang
Abstract
Jichun Wang
Abstract
Sequence related amplified polymorphism(SRAP) markers were employed to analyze the genetic diversity of potato(Solanum tuberosum) varieties. Twenty three out of the randomly selected 27 primer pairs were polymorphic among the 44 varieties tested, and the polymorphism ratio of primers was 85.2%. A hundred and four polymorphism bands were obtained, and the average polymorphism bands were 4.5 per primer pairs. These results demonstrated that SRAP had higher polymorphism in the tested potato varieties. Based on cluster analysis with the SRAP makers, the genetic distance range among the tested 44 varieties was from 0.147 to 0.741. At the genetic distance of 0.67, 44 varieties were clustered into 4 main groups, including one compound group and three discrete ones. Furthermore, the compound group could be subdivided into 7 subgroups. The genetic diversity of the tested potato varieties was thus confirmed at the DNA level.
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Sequence related amplified polymorphism(SRAP) markers were employed to analyze the genetic diversity of potato(Solanum tuberosum) varieties. Twenty three out of the randomly selected 27 primer pairs were polymorphic among the 44 varieties tested, and the polymorphism ratio of primers was 85.2%. A hundred and four polymorphism bands were obtained, and the average polymorphism bands were 4.5 per primer pairs. These results demonstrated that SRAP had higher polymorphism in the tested potato varieties. Based on cluster analysis with the SRAP makers, the genetic distance range among the tested 44 varieties was from 0.147 to 0.741. At the genetic distance of 0.67, 44 varieties were clustered into 4 main groups, including one compound group and three discrete ones. Furthermore, the compound group could be subdivided into 7 subgroups. The genetic diversity of the tested potato varieties was thus confirmed at the DNA level.
Key concepts: Biology, Genetic diversity, Germplasm, Solanum tuberosum, Polymorphism (computer science), Primer (cosmetics), Genetic distance, Genetics