Effects of Resveratrol on the expression of TGF-β1 in rat mesangial cells under high glucose

Objective:To investigate the effect of Resveratrol(Res)on the expression of high glucose-activated transforming growth factor-β1(TGF-β1) in rat renal mesangial cells.Methods:①Cultured rat renal mesangial cells were exposed to D-glucose at 30 mmol /L for periods of 24,48,72 h,respectively.Normal glucose(5.6 mmol /L) served as control.②Cultured rat renal mesangial cells were divided into four groups:normal glucose(5.6 mmol /L),high glucose(30 mmol /L),normal glucose plus Resveratrol(20 μmol /L),high glucose plus Resveratrol at 5,10,15,20 μmol /L,respectively for periods of 48 h.The mRNA levels of TGF-β1 were measured by RT-PCR and the protein levels of TGF-β1 were detected by Western Blotting.Results:As compared to normal cells,exposure of rat renal mesangial cells to D-glucose at concentrations of 30 nmol /L caused a time-dependent increase in the mRNA and protein levels of TGF-β1 and reached the highest level at approximately 48 h.The addition of Resveratrol significantly attenuated high glucose-enhanced mRNA and protein levels of TGFβ1(P 0.05 or P 0.01) in a dose-dependent manner.No notable difference at the mRNA and protein levels of TGF-β1 was found between Res and NC group(P 0.05).Conclusion:High glucose contributes to an increasing of TGF-β1 expression at both the mRNA and protein levels in rat renal mesangial cells.Resveratrol can significantly reverse high glucose up-regulated expression of TGF-β1 in the rat renal mesangial cells and may play a role in the treatment of diabetic nephropathy.