Expression of DOG1 and WISP-1 in gastrointestinal stromal tumors of 124 cases and their clinic significance

Purpose To investigate expression and clinical significance of DOG1 and WISP-1 in gastrointestinal stromal tumors(GISTs).Methods One hundred and twenty-four GISTs with complete clinicopathologic data were retrieved from archival files of department of pathology,Affiliated Hospital of North Sichuan Medical College.The expression levels of DOG1 and WISP-1 were detected by immunohistochemistry in 124 cases of GIST tissues,and specimens from non-GISTs were used as controls.Statistical analysis were performed using χ2 test and Spearmen test.Result Of 124 GISTs,119(96%) were positive for DOG1 and 111(91.0%) for CD117.DOG1 protein expression rate was significantly different between GISTs and non-GISTs(P0.001),but there were no significant differences between DOG1 and CD117 expression in GISTs.As for WISP-1,this antibody yielded positive staining in 100 of 124(80.6%) GISTs.The expression rate of WISP-1 protein in very low risk,low risk,intermediate risk,and high risk cases was 44.4%(4/9),66.7%(26/39),86.9%(17/20),95.0%(38/40),and there was a significant correlation between WISP-1 protein expression and risk score proposed by NIH(2008).Conclusions DOG1 is a sensitivity and specificity marker for GIST and should be used as the first line antibody as CD117 protein in routine diagnosis.The expression of WISP-1 maybe associated with GIST progression and could be used as a prognosis marker in GIST.