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2011•Sichuan Nongye Daxue xuebaoRequires access

Cloning and Verification of ATGL Gene in Mini-pig and Comparison of the Coding Sequence in Different Porcine Breeds

Changxin Wu

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Abstract

ATGL gene was recently identified as a novel gene and its expression product was named as adipose triglyceride lipase(ATGL),which was proved to be the rate-limiting enzyme that catalyse the initial step in triacylglycerol hydrolysis.A total of 2 102 bp cDNA sequence of ATGL gene was cloned by using the electronic cloning and RT-PCR methods from Guizhou mini-pig in this study.The coding region of ATGL gene cDNA was 1 461 bp,which encoded 486 amino acids.Sequencing analysis indicated that the cDNA sequence of ATGL gene and the predicted protein sequences were highly conserved among different porcine breeds.

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What this paper is about

ATGL gene was recently identified as a novel gene and its expression product was named as adipose triglyceride lipase(ATGL),which was proved to be the rate-limiting enzyme that catalyse the initial step in triacylglycerol hydrolysis.A total of 2 102 bp cDNA sequence of ATGL gene was cloned by using the electronic cloning and RT-PCR methods from Guizhou mini-pig in this study.The coding region of ATGL gene cDNA was 1 461 bp,which encoded 486 amino acids.Sequencing analysis indicated that the cDNA sequence of ATGL gene and the predicted protein sequences were highly conserved among different porcine breeds.

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Available abstract

ATGL gene was recently identified as a novel gene and its expression product was named as adipose triglyceride lipase(ATGL),which was proved to be the rate-limiting enzyme that catalyse the initial step in triacylglycerol hydrolysis.A total of 2 102 bp cDNA sequence of ATGL gene was cloned by using the electronic cloning and RT-PCR methods from Guizhou mini-pig in this study.The coding region of ATGL gene cDNA was 1 461 bp,which encoded 486 amino acids.Sequencing analysis indicated that the cDNA sequence of ATGL gene and the predicted protein sequences were highly conserved among different porcine breeds.

Key concepts: Adipose triglyceride lipase, Cloning (programming), Gene, Complementary DNA, Coding region, Biology, Sequence (biology), Sequence analysis

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