The analysis on the flow cytometry immunity result for 51 adult cases with acute leukemia

Objective:To explore the clinical value of flow cytometry immunity result for acute leukemia(AL).Methods:The immunophenotypes and morphologic examination of bone marrow collected from 59 patients with AL were analyzed by four antibody combinations of multiparametric flow cytometry and cytochemistry staining,respectively.Results:Antigens including CD33,HLA-DR,CD13,MPO and CD117 were primarily expressed in 43 patients with acute myeloid leukemia(ALL),the specificity of MPO and CD117 was higher;CD7 was expressed in 18.64% patients(Ly+AML),and CD19 was expressed in 10.16% patients(Ly+AML).Antigens including CD79a,HLA-DR,CD19,CD20 and CD10 were primarily expressed in 12 patients with acute lymphocytic leukemia,the specificity and sensitivity of CD79a were higher;CD13 was expressed in 8.47% patients.CD34 was dimly expressed and HLA-DR was undetectable in patients with acute promyelocytic leukemia.4 patients were diagnosed by flow cytometer,which couldn′t accurately classified by morphologic examination.Conclusion:By combining immunophenotypes and morphologic examination of bone marrow,diagnostic accuracy of adult acute leukemia may be improved,was help to treat and predict prognosis of patients with acute myeloid Leukemia,and to define some special types of leukemia.