2005Guoshu xuebaoRequires access

A Study on the factors effecting the adventitious shoot regeneration from hypocotyls of wild apricot

Qingmei Guan

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Abstract

Hypocotyls rasied from mature seeds of Prunus armeniaca L var. ansu cultured on modified MS medium were cut into segments and cultured on the regeneration medium, which consisted of modified MS + TDZ 2.0 mg/L+NAA 0.5 mg/L, in dark for 15 d and then exposed to light. The rate of shoot regeneration was higher than 37%. The result showed that TDZ was better than 6-benzylamino-purine (6-BA), and NAA was better than 2,4-D in adventitious shoot regeneration. 2 to 3 week-aged hypocotyls produced better result, but the position effect of explants was not significant for shoot regeneration and callus induction.

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What this paper is about

Hypocotyls rasied from mature seeds of Prunus armeniaca L var. ansu cultured on modified MS medium were cut into segments and cultured on the regeneration medium, which consisted of modified MS + TDZ 2.0 mg/L+NAA 0.5 mg/L, in dark for 15 d and then exposed to light. The rate of shoot regeneration was higher than 37%. The result showed that TDZ was better than 6-benzylamino-purine (6-BA), and NAA was better than 2,4-D in adventitious shoot regeneration. 2 to 3 week-aged hypocotyls produced better result, but the position effect of explants was not significant for shoot regeneration and callus induction.

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Available abstract

Hypocotyls rasied from mature seeds of Prunus armeniaca L var. ansu cultured on modified MS medium were cut into segments and cultured on the regeneration medium, which consisted of modified MS + TDZ 2.0 mg/L+NAA 0.5 mg/L, in dark for 15 d and then exposed to light. The rate of shoot regeneration was higher than 37%. The result showed that TDZ was better than 6-benzylamino-purine (6-BA), and NAA was better than 2,4-D in adventitious shoot regeneration. 2 to 3 week-aged hypocotyls produced better result, but the position effect of explants was not significant for shoot regeneration and callus induction.

Key concepts: Hypocotyl, Shoot, Callus, Regeneration (biology), Explant culture, Prunus armeniaca, Basal shoot, Biology

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