Efficient Adventitious Shoot Regeneration in Cicer Milkvetch

A procedure has been developed for high frequency adventitious shoot regeneration from hypocotyls, cotyledon, stem and petiole explants of cicer milkvetch. All explants isolated from in vitro seedlings were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 6-benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA) to induce adventitious shoot regeneration. Hypocotyl explants appeared to have better regeneration capacity than stem, cotyledon and petiole explants in most of the media tested. The highest frequency of shoot regeneration was achieved from hypocotyl segments through an initial callus growth on MS medium containing 10 μM BA and 0.1 μM NAA. Regenerated shoots were excised and rooted in half-strength MS medium supplemented with 5.4 μM NAA. Rooted plantlets were acclimatized to ambient conditions and later established under greenhouse conditions.