HPLC Method for Simultaneous Determination of Baicalin,Baicalein,Aloe Emodin and Emodin in Niuhuang Jiedu Tablets

Objective: To establish a method for the determination of multi- components( baicalin,baicalein,aloe emodin and emodin) in Niuhuang Jiedu tablets( NHJDT) and perfect the quality control standards. Methods: Using Agilent ZORBAX SB- C18( 4. 6 × 250 mm,5μm) as the separation column,the UV absorbance was monitored at 260 nm by injecting 10μL of the sample solution each time and the column temperature was maintained at 30℃. The flow rate was1. 0m L / min. Acetonitrile was as the mobile phase A; 0. 1% phosphoric acid was as mobile phase B for gradient elution.Results: The linear range in content determination of baicalin,baicalein,aloe emodin and emodin was followed by5. 06 ~ 202. 4μg / m L,1. 836 ~ 30. 6μg / m L,0. 196 ~ 7. 84μg / m L and 0. 992 ~ 19. 84μg / m L,the correlation coefficient R2 was in turn 0. 999 7,0. 999 1,0. 999 8 and 0. 999 8. The average recoveries were 99. 17%( RSD = 0. 72%),97. 46%( RSD = 0. 85%),97. 91%( RSD = 1. 97%),98. 23%( RSD = 0. 82%)( n = 6). Conclusion: The method is accurate and can be used to evaluate the quality of NHJDT.