Preparation and in vitro release of CA4-loaded solid lipid nanoparticles

OBJECTIVE To prepare the CA4-loaded solid lipid nanoparticles(CA4-SLNs) and investigate its physicochemical properties and in vitro release profile.METHODS CA4-SLNs were prepared by the method of emulsification evaporation-solidification at low temperature.The morphology was examined by transmission electron microscope.The particle size and ξ potential were determined by laser granularity equipment.The unencapsulated CA4 and solid lipid nanoparticles were separated by centrifugation and the encapsulation efficiency and drug-loading efficiency were detected by HPLC.The in vitro release profile of CA4-SLNs was studied using dialysis technology.RESULTS The obtained SLNs were spherical.The mean particle size was 73.23 nm.The PDI was 0.238,and the ξ potential was-30.5 mV.The average encapsulation efficiency and drug loading of the optimized SLN were 98.62% and 3.89%.The release in vitro accorded to the Weibull order model:lnln[1 /(1-Q)]= 0.6123lnt-0.736(r = 0.9917).CONCLUSION The method of emulsification-evaporation was appropriate for the preparation of CA4-loaded solid lipid nanoparticles and the drug encapsulation efficiency was high.The in vitro release test showed that the drug could sustained-release from SLN after a burst release in initial phase.