The Comparative study and bias estimation on the results of serum cardiac marker enzyme with different biochemical testing system inter-hospital

Objective To study the comparability results of cardiac marker enzyme AST,CK、LDH and α-HBDH on different biochemical detecting systems inter-hospital through bias estimation and method comparison.Methods Following to CLSI document EP9-A,we applied native blood samples and tested in different selfbuilt biochemical testing systems and aim system respectively to obtain the correlation coefficient and the linear equation.The comparability of different investigating systems were judged according to the half of CLIA'88 standard.Results 1.the correlation coefficient of LDH(system 2) was less than 0.975 were not acceptable,the rest were acceptable.2.the bias rate of CK(system 4,6),α-HBDH(system 3,4) in normal and un-normal level and CK(system 2),α-HBDH(system 6) in un-normal were higher than the half of CLIA'88 standard were not acceptable,the rest were acceptable.Conclusion There were several items were not acceptable in self-built system and it is necessary to do method comparison and bias estimation for judge clinical acceptability in order to insure the comparability.