Research on Hydrolyzation Conditions of Digesting Meretrix meretrix L. by Several Proteinases

Trypsin and papain were selected from trypsin,papain,pepsin and flavor-enzyme,when the degrees of hydrolysis were measured in digesting Meretrix meretrix L.by those proteinases.Furthermore,better technics of trypsin,papain and these two combination in hydrolyzation of Meretrix meretrix L.were studied.The results indicated that the effect of these two combination on hydrolysis was the best.The best technics was that the 6000 u/g trypsin was added first for the hydrolisis for 6 h in the condition of 50 ℃,pH 8.0 and substrate concentration 1∶3(v /w),and then 2000 u/g papain was added for the hydrolisis for 2 h in the condition of 55 ℃,pH 5.5 and substrate concentration 1∶3(v/w).On those conditions,the combination between trypsin and papain had good effect on digesting Meretrix meretrix L.and the hydrolysis degree was 28.15%.