2012•Journal of Zhangzhou Normal UniversityRequires access
On Preparation of Peptides from Meretrix Meretrix Linnaeus by Parenzyme Enzymolysis Method
Hang Fu-zi
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Abstract
In this present,Optimum hydrolysis conditions of Parenzyme to the Meretrix Meretrix Linnaeus are studied.Several factors such as pH,temperature,time and enzymes dosage of the hydrolysis are determined by single factor experiments and orthogonal experiment.The results show that optimal hydrolysis conditions of Parenzyme to the Meretrix Meretrix Linnaeus are respectively pH 8.5,temperature of 50 C,enzymes dosage of (87500:1) U / g,reaction time of 5h,and peptides of low molecular weight of 500-3500 Da can be prepared effectively.