A CONTRASTIVE STUDY ON INFLUENCE BETWEEN INTERVENTION WITH RECOMBINANT HUMAN ERYTHROPOIETIN OR METHYLPREDNISOLONE AFTER ACUTE SPINAL CORD INJURY IN RATS

Objective:To observe contrastively the neuroprotective effect and the change of IL-10 expression in spinal cord tissue by intervention with rHuEPO or MP after acute spinal cord injury in rats.Methods:Acute spinal cord injury models were created in adult Wistar rats with improved Allen's weight drop methods(36g cm).The rats were randomly divided into three groups after acute spinal cord injury:SCI group,rHuEPO group and MP group.Neurological function were evaluated by detecting inclined plane angle and motor evoked potential(MEP)at 28th day after spinal cord injury in each group.HE staining and immunohistochemistical staining of IL-10 of spinal cord tissue were also performed.Results:The expression of IL-10 and inclined plane angle and the amplitude of motor evoked potential was significantly increased in rHuEPO group and MP group compared with SCI group.The pathological changes in spinal cord tissue were significantly improved in rHuEPO group and MP group than in SCI group.Conclusion:rHuEPO could improve IL-10 expression in acute spinal cord injury rats,and its effect was not better than MP's.They also could protect the neurological function after rat's acute spinal cord injury.