2004•Unpublished venueRequires access
Effect of fluoride on morphologic changes of cell columns and apoptosis of new-born rabbit in vitro
Chen Yan-pin
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Abstract
Objective To observe the direct influence of fluoride on rib cartilage of new-born rabbit in vitro, then discussing the effect of fluoride on morphologic changes of cell columns and apoptosis of cartilage cells. Methods The coastal cartilages of newborn-rabbits were cultured with fluoride in various concentration (20?320 μmol/L) with/without SOD(0.006 U/L) for 18days. Then examined: morphologic changes with image analysis and ultra-structural changes;apoptosis was detected with terminal deoxyribonucleotide transferase-mediated dUTP-bintin(TUNEL) method; and the concentration of H2O2 and NO in the culture medium were tested. Results ①High concentration fluoride treated the chondrocytes were proliferated, cell columns being longer but thinner when being compared with the control, the perimeter, area and length of cartilage columns increased, but the cross diameter decreased in the two fluoride treated groups. The above-mentioned changes were markedly alleviated in the two fluoride plus SOD groups. It was observed the characteristics of apoptosis in every fluoride-treated groups, but it was serious in 320 μmol/L group than other groups. ②The AI (%) of fluoride-treated groups (320 μmol/L) was remarkably high as being compared with the control (P 0.01),the AI value of SOD plus fluoride decreased significantly when compared with fluoride-treated groups (P 0.05,P 0.01). ③The H2O2 content in the two groups-treated with high concentration fluoride increased markedly when compared with the control (P 0.05, P 0.01);the NO content in treated groups was markedly higher than that of the control(P 0.01, P 0.05) except for low concentration fluoride plus SOD group. Conclusions ①Fluoride causes the changes of cell columns of cartilage and damages the cell ultrastructure. ②In vitro, fluoride induced apoptosis of new-born rabbit cartilage chondrocytes and SOD can inhibit it. ③Excessive free radicals partly responsible for the mechanisms of fluoride induced apoptosis.