Effect of protein expression of Ras gene in osteoblasts cultured in vitro and exposed to fluoride

Objective: To study effect of protein expression of Ras gene in osteoblasts cultured in vitro and exposed to different does of fluoride. Methods: Osteoblasts were obtained from the calvaria of mice within 1～3 day by enzymic digestion method and were cultured. Then osteoblasts were exposed to different does of fluoride. Detected protein expression of Ras of mice osteoblasts exposed different does of fluoride by immunohistochemistry and Western blotting. Results: All groups had protein postitive expression after exposed to fluoride, but the 2.5 mg/L fluoride groups of postitive degree and area were stonger and bigger than controal group and the rest fluoride groups of postitive degree and area were weaker and smaller than controal group. Calculation of postitive osteobalsts numbers showed that it had statistic difference between the fluoride groups and the controal group. The numbers of 2.5 mg/L fluoride groups were higher than the control group (P0.05) and the numbers of the rest fluoride groups were lower than the control one (P0.05). Among of these number of 2.5 mg/L fluoride group was the highest. The correspondence quantitation result of protein of Ras of mice showed that the numbers of 2.5 mg/L and 5mg/L fluoride groups were more than the control group and the numbers of the rest fluoride groups were less than the control one,but the fluoride groups had decreased tendency with increase the fluoride dose. Conclusions: Fluoride influence the protein expression of Ras of osteoblasts,which shows double action:low does of fluoride improves and high does of fluoride inhibits.