Effect of Fluoride on Expression of P53 in Human Embryo Hepatocytes

[Objective] To investigate P53 expression induced by fluoride in human embryo hepatocytes.[Methods] After L-02 cells were incubated with 40,80,and 160 μg/ml sodium fluoride for 24 hours,the cell survival rate,the level of P53 protein and mRNA expression was assessed by MTT,Western blot and RT-PCR analysis respectively.[Results] The cell survival rate in L-02 cells treated by high concentration of fluoride was significantly lower than that in the control group.The expression levels of P53 protein in middle and high concentration fluoride group were obviously higher than that in the control group,and increased as the concentration of fluoride increased.Although no significant difference was seen between the low concentration fluoride group and the control group,there was a rising tendency.The P53 mRNA levels in all fluoride groups were markedly higher than that of the control group.In the low concentration group and the medium concentration group,the levels of P53 mRNA were increased with the increasing concentration of fluoride.The P53 mRNA expression of the high concentration fluoride group was statistically different from that of the control group,but compared with the medium concentration group,it seemed to go down,and it's similar to the low concentration fluoride group.[Conclusion] Fluoride can decrease the cell survival rate and induce P53 expression in L-02 cells,but there was no consistent tendency between the expression of P53 mRNA and protein.