Distribution and drug resistance analysis of clinically isolated 531 strains of gram-negative bacilli

【Objective】To investigate the distribution and drug resistance of gram-negative bacilli from the clinical specimen,for the rationalized use of antibiotics in clinical practice.【Methods】Anti-microbial susceptibility test was done on 531 strains of gram-negative bacilli isolated in our hospital from January 2005 to December 2005 by Kirbybauer method,the detection of ESBLs-producing strains was performed for screening and confirming the presence of ESBLs.【Results】The primary proportion of gram-negative bacilli was Escherichia coli 36.3%,Pseudomonas aeruginosa 23.0%,Klebsiella pneumoniae 14.7%.The susceptibility of these strains were different in 12 antibacterials.Imipenem was the most active antibiotics against gram-negative bacilli,cefoperazone/sulbactam and piperacillin/tazobactam also showed excellent activity.ESBLs producing strains were detected in 26.9% of Escherichia coli and 38.5% of Klebsiella pneumoniae,the susceptibility was remarkablly lower than those of ESBLs negative strains.【Conclusions】Drug resistance of gram-negative bacilli was high and multiple in our hospital.The importance of detecting extended ESBLs occurrence in clinical lab,helping rationalize antibiotic use in clinical practice.