Antibiotic resistance monitoring among 1939 Gram-negative bacilli strains

Objective To investigate the drug resistance of Gram-negative bacilli isolated from the clinical specimen in Guangzhou Oversea Chinese Hospital from 2007 to 2009,so as to guide the reasonable using of antibiotics in clinical practice.Method The bacteria were identified with Bio-Merieue VITEK-2 Compact system and tested with K-B method.Whonet 5.4 software was used to analyze the results of drug sensitivity tests.Result Of the 1 939 Gram-negative bacilli isolated,the top three were Pseudomonas aeruginosa(479 strains),Esherichia coli(452 strains) and Acinetobacter baumannii(257 strains).Most of the strains were isolated from Department of Respiratory Medicine and ICU,mainly from sputamentum samples.The prevalence of ESBLs in E.coli,Klebesiella pneumoniae and Proteus mirabilis were 54.8%,43.9% and 37.5%,respectively.ESBLs non-producing strains showed lower resistance rates to sulbactam/cefoperazone,trimethoprim/sulfamethoxazole,aminoglycosides,quinolones,penicillin/enzyme inhibitor,and the third and fourth generation cephalosporin than the ESBLs producing strains.Nonfermentative gram negative bacilli were very highly resistant to most antibiotics(31.0%~74.0%),while sensitive to sulbactam/cefoperazone and carbapenem.And multidrug resistant strains were detected.Conclusion The antibiotic resistance of the 1 939 gram negative bacilli is critical,with ESBLs producing strains and multidrug resistant strains having been detected.It is very important to select antibiotics correctly according to the results of susceptibility test,which can offer first-hand epidemiology information and dynamic changes of antibiotic resistance for clinical practice.