2010•Chinese Journal of Animal Infectious DiseasesRequires access
EXPRESSION OF ANTIBIOTIC PEPTIDE OF GENE M50 IN BAC-TO-BAC BACULOVIRUS SYSTEM
Siqi Yang
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Abstract
The M50 Gene encoding antibiotic peptide of tick Rhipicephalus haemaphysaloide was cloned into the Baculovirus transfer vector pFastBacHTA.The recombinant plasmid pFastBacHTA-M50 was transformed into competent cells of E.coli DH10Bac.The purified recombinant shuttle plasmid was transfected into sf9 insect cells to express recombinant protein M50.The recombinant protein expressed was detected by Western blot and immunofluorescent assay.The results showed that M50 protein was successfully expressed in the Bac-To-Bac Baculovirus system.The fusion M50 protein with His tag(His-M50) had a molecular weight of 15kDa.His-M50 was recognized by anti-His monoclonal antibody by Western blot and was positive stained with mouse anti-serum against M50 protein.