Bac-to-Bac Baculovirus Expression System in IL-2 Protein of BHK

Objective:To construct recombinant mouse IL-2 protein in BHK cells using Bac-to-Bac baculovirus expression system.Methods:The mouse IL-2 was marked by Flag,and joined the eukaryotic cell promoter CMV.The plasmid pFB-CMV-IL-2 was constructed to recombinant,restriction enzyme was used to digest,and was amplified by PCR.The mouse IL-2 was cloned into the baculovirus expression vector pFastBacDual and pFB-CMV-IL-2 was transformed into baculovirus shuttle vector containing the DH10Bac competent bacmid bacteria.The clones were recombinant baculovirus vector Bacmid-pFB-CMV-IL-2,extracted plasmid with liposome transfection method Bacmid-pFB-CMV-IL-2 transfected Sf9 insect cells packaging the virus,harvested recombinant baculovirus.A virus transduction of BHK cell culture was built using Western blot to detect the IL-2 protein.Results: By baculovirus expression system,mouse recombinant IL-2 protein was successfully expressed in BHK cells.Conclusion: Recombinant baculovirus was successfully expressed mouse IL-2 protein in eukaryotic cells.