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Determination of the Content of Polysaccharide in Walnut Shell

Zhou Ping

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Abstract

[Objective] To determine the content of polysaccharide in walnut shell.[Method] The content of polysaccharide was determined by phenol-sulfuric acid method at 490 nm with the glucose as reference substance.[Result] The optimal color conditions were 1.0 ml 5% phenol,6.0 ml sulfuric acid,65 ℃ reaction temperature and 30 min reaction time,the average recovery was 101.9%,RSD=1.98% and the liner range was 10-90 μg/ml.[Conclusion] The method is simple,accurate and has good reproducibility,and it is reliable to determine the total polysaccharide in the walnut shell.

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What this paper is about

[Objective] To determine the content of polysaccharide in walnut shell.[Method] The content of polysaccharide was determined by phenol-sulfuric acid method at 490 nm with the glucose as reference substance.[Result] The optimal color conditions were 1.0 ml 5% phenol,6.0 ml sulfuric acid,65 ℃ reaction temperature and 30 min reaction time,the average recovery was 101.9%,RSD=1.98% and the liner range was 10-90 μg/ml.[Conclusion] The method is simple,accurate and has good reproducibility,and it is reliable to determine the total polysaccharide in the walnut shell.

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Available abstract

[Objective] To determine the content of polysaccharide in walnut shell.[Method] The content of polysaccharide was determined by phenol-sulfuric acid method at 490 nm with the glucose as reference substance.[Result] The optimal color conditions were 1.0 ml 5% phenol,6.0 ml sulfuric acid,65 ℃ reaction temperature and 30 min reaction time,the average recovery was 101.9%,RSD=1.98% and the liner range was 10-90 μg/ml.[Conclusion] The method is simple,accurate and has good reproducibility,and it is reliable to determine the total polysaccharide in the walnut shell.

Key concepts: Polysaccharide, Sulfuric acid, Phenol, Chemistry, Reproducibility, Chromatography, Shell (structure), Content determination

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