2008•Food ScienceRequires access

Study on Phenol-Sulfuric Acid Method for Determination of Polysaccharide Content in Pleurotus eryngii

Jin-Long Guo, Chen You-Jun, Sun Guo-Qin, Guo Jiu-Feng, Yunhua Wang, Zhu Xiao-qing, Nie Qi-Sen

Abstract

The factors affacting the determination of polysaccharide content in Pleurotus eryngii with phenol-sulfuric acid method were optimized. Results showed that under the conditions of detection wavelength 485 nm,phenol content 1.4 ml,reaction temperature 40 ℃ and reaction time 15 min,there is good linearity range from 10.00 to 70.00μg/ml(r=0.9996). The average recovery is 99.2% and the RSD is 1.16%.

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Key concepts: Pleurotus eryngii, Chemistry, Phenol, Polysaccharide, Sulfuric acid, Chromatography, Content determination, Food science