Studies on Microspore Culture and Doubling Efficiency in Spring-Planted Brassica napus

Studies on microspore culture of a set of F1 hybrids of spring-planted Brassica napus and their parents.The results indicated that the difference of ratio of embryos among different genotypes was much great.In all of the 22 donor materials,there were 21 of them being obtained embryos in 2006,average embryos per bud of 40.91% and 31.82% of which were over 5 and 10,respectively.The ratios of embryos of most F1 hybrids and the terminal raceme were higher than their parents′ and primary branches′,respectively.In the treatments of 0.6 g/L colchicines for 5.0 h and 3.4 g/L colchicines for 1.5h,the efficiencies of survival and doubling were the highest.There were fertile plants and semi-fertile plants after doubling,the pollen vigor of the former was higher than the latter′s,furthermore,the selfing seed-set index of the former was high,too.And the selfing seed-set index of 50% semi-fertile plants was lower than 1.