Studies on Microspore Culture and Double Haploid Breeding on Rape II.Influencing Factors on Microspore Culture of F_1 Hybrids between Brassica napus and Brassica juncea

The microspore culture of the interspecific F 1 hybrids between Brassica napus and Brassica juncea was investigated. The results showed: (1) The embryo output of the F 1 hybrids was related to the genotype of their parents; (2) The pollen fertility of the F 1 hybrids with a rate from 30% to 43% had no obvious effect on the efficient embryo output; (3) The donor plant age had obvious effect on those hybrids with lower embryo yield and no much effect on those with higher yield; (4) The anther color with yellowish green was the most suitable for the induction of embryogenesis; (5) It was better to transfer the embryo cultures from liquid NLN to semisolid B 5 medium for 3 days and then to the solid B 5 medium increasing regeneration as comparing with the transfer from the liquid directly to the solid ; (6) The embryos with 27 days in age and 0.2 cm to 0.3 cm in size were ideal explants for getting the regeneration.