Optimization of Purification Process for Total Flavonoids from Propolis by Macroporous Resin

[Objective]To optimize Purification technology conditions of total flavonoids from propolis by macroporous adsorption resin.[Method]With static adsorptioncapacity and static desorption rate as indexes,optimum type of macropo- rous resin was selected by comparing adsorption and desorption effect of 4 kinds macroporous resin for total flavonoids from propolis.Then,the optimized resin were investigated for its property of dynamic absorption and property of dynamic desorption,including the sample concentration,absorption flow rate,eluting agent,elution flow rate and the absorption time.[Result] FL-1 macroporous resin was the most suitable for purification of total flavonoids from propolis .Optimum purification conditions were: the concentration of sampling liquid of 0.25～0.38 mg/mL,sampling velocity 1mL/min,adsorption time 1h,eluted by 90% ethanol at the speed of 1 ml/min,eluate was collected. Under these process conditions,yield of total flavonoids was from 13.07% to 85.25% ,recovery for total flavonoids was 79.37%.[Conclusion] FL-1 macroporous resin had a good purification effect of total flavonoids from propolis,optimized purification process wa sreasonable,stable and feasible.