The Paraffin Section Technique of the Remaining Tissue after Frozen Section

Objective To compare the effects of low temperature fixation ordinary temperature fixation on freezing tissue. Methods The remaining anolonely tissue (colon and stomach) after frozen section of 48 SD rats were randomly divided into 2 groups:low temperature fixation (LTF) and ordinary temperature fixation (OTF). LTF was treated by 4 ℃4% paraformaldehyde for 1 hour,fixated in room temperature for 12 hours,dehydration and paraffin imbedding by routine method. OTF was fixation in room temperature,dehydration and paraffin imbedding by routine method. Both groups were stained by hematoxylin-eosin staining,oluidine blue staining and immunohistochemical staining methods respectively,being observed by microscope. Results It was shown that the cellular stucture was clearer in LTF than that in OTF. The colour of the paraffin section was bright in LTF. The contrast of the nuclear and cytoplasm in LTF was clear. We could see more spacing caused by ice crystal in paraffin section of OTF. Conclusion There is no obviously differences in immunohistochemical slices.The study indicates that the effect of low temperature fixation of the freezing tissue of paraffin section is better than that of ordinary temperature fixation.