The Effects of Fluoride on ALP Activity in Cultured Human Dental Papilla Mesenchymal Cells

To understand the effects of fluoride on ALP activity in cultured human dental papilla mesenchymal (HDPM) cells and the relationship between cell activity and ALP activity. Cultured HDPM cells were treated with NaF of 0μmol,10μmol (0.2×10 -6 g/L),52μmol(1.0×10 -6 g/L),263μmol (5.0×10 -6 g/L),1 315μmol(25×10 -6 g/L),2 630μmol(50×10 -6 g/L),respectively. The activities of secreted ALP and cells were measured by enzyme kinetic method and 3 [4,5 Dimothylthiazol 2 y] 2,5 diphenyltetrazolium bromide(MTT).The results showed that 263μmol(5.0×10 -6 g/L)of NaF in vitro test for 24h inhibited the activity of HDPM cells while the activity of secreted ALP was not inhibited; 1 315μmol (25×10 -6 g/L) and 2 630μmol(50×10 -6 g/L)for 12h the activity of HDPM cells was inhibited whereas the activity of secreted ALP was increased;2 630μmol for 72h,in spite of the fact that nearly all cells were died, the activity of ALP was still higer than that of the control. It suggested that the level of ALP was not accordant with the activity of HDPM cells when treated by fluoride.