Study on Extraction Methods of Soybean Genomic DNA

[Objective] The study was to extract high quality and sufficient yield of genomic DNA of soybean and to provide a reference for the extraction genomic DNA of soybean.[Method] With soybean seed as material,genomic DNA was extracted by SDS、CTAB and high salt and low pH value method.The genomic DNA was determined through optical density,ultraviolet spectra,agarose gel electrophoresis and the restriction enzyme digestion of DNA.[Result] The results of optical density and spectrum detection showed that,extraction amount and purity with SDS method were better than CTAB method and high salt and low pH value method;The results of agarose gel electrophoresis determination showed that,compared with genome DNA band extracted by high salt and low pH value method,genome DNA band was more crude,brighter and better repeatability extracted by SDS method;The results of restriction endonuclease determination showed that genome DNA obtained from three method can be restriction enzyme by HindⅢ and EcoRⅠ,and SDS method was best for the effect of genome DNA of restriction enzyme.