Study on Extraction and Purification Methods of Soybean Genomic DNA

[Objective] The study was to improve the purity and quality of soybean genomic DNA with gradually enriching method and to provide a reference for the extraction and purification of other animal and plant genomic DNA.[Method] With Meiqiao soybean as material,soybean genomic DNA were purified on the basis of extracting soybean genome DNA by CTAB method.The purified DNA was determined through optical density,ultraviolet spectra,agarose gel electrophoresis and RAPD-PCR,etc.[Result] The detection by optical density,ultraviolet spectra showed that A260/A230 was from 1.678 to 1.722 with an average of 1.697,and the A260/A280 was from 1.733 to 2.022 with a mean value of 1.844.The agarose gel electrophoresis showed the soybean genomic DNA had greater molecular weight,well integrity and little RNA residue.The clear and rich polymorphism bands were obtained through the detection of RAPD-PCR reaction.The yield of soybean genomic DNA was 66.969 μg/g.The method studied by this experiment could get the soybean genomic DNA with high quality and had the characteristic of simple operation and not high requirement of experimental condition.[Conclusion] The method could be applied to purify DNA of other animal and plants.