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Production of nuclease by Penicllium citrinum fermentation

Xiaoli Zhang

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Abstract

Penicillium citrinum W48,a high yield strain which is mutated with ultraviolet radiation rays and 60Co radiation was cultivated.The constitutes of the fermentation culture and fermentation conditions was optimized.The optimum culture contains:KH2PO4 0.05%,K2HPO4 0.03%,yeast extract + peptone 0.7%,CaCl2 0.02%,MgSO4 0.04%,ZnSO4 0.03%,Glucose 5%,pH 5.5.The optimum cultivated conditions are inoculation quantity 10%,liquid volume 50 mL,rotation speed 180r/min,fermentation temperature 30 ℃,time 66 h.

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What this paper is about

Penicillium citrinum W48,a high yield strain which is mutated with ultraviolet radiation rays and 60Co radiation was cultivated.The constitutes of the fermentation culture and fermentation conditions was optimized.The optimum culture contains:KH2PO4 0.05%,K2HPO4 0.03%,yeast extract + peptone 0.7%,CaCl2 0.02%,MgSO4 0.04%,ZnSO4 0.03%,Glucose 5%,pH 5.5.The optimum cultivated conditions are inoculation quantity 10%,liquid volume 50 mL,rotation speed 180r/min,fermentation temperature 30 ℃,time 66 h.

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Available abstract

Penicillium citrinum W48,a high yield strain which is mutated with ultraviolet radiation rays and 60Co radiation was cultivated.The constitutes of the fermentation culture and fermentation conditions was optimized.The optimum culture contains:KH2PO4 0.05%,K2HPO4 0.03%,yeast extract + peptone 0.7%,CaCl2 0.02%,MgSO4 0.04%,ZnSO4 0.03%,Glucose 5%,pH 5.5.The optimum cultivated conditions are inoculation quantity 10%,liquid volume 50 mL,rotation speed 180r/min,fermentation temperature 30 ℃,time 66 h.

Key concepts: Penicillium citrinum, Fermentation, Food science, Yeast, Volume (thermodynamics), Chemistry, Yield (engineering), Strain (injury)

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