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Studies on the Culture Conditions of Phytase Producing Penicillium P11

Yan Liu

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Abstract

The effect of the various carbon and nitrogen sources on fermentation of Penicillium P11 that produces phytase was studied.The results indicated that the best carbon and nitrogen compounds for this strain were sucrose,peptone and ammonium nitrate respectively.The optimum producing condition was cultivate temperature at 30℃,pH of the fermentation medium at 5;medium quantity,10%;inoculation proportion,4%;rotate speed of the shaker,120r/min;fermentation time,96h.The highest enzyme activity could reach 2.63U/mL.

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What this paper is about

The effect of the various carbon and nitrogen sources on fermentation of Penicillium P11 that produces phytase was studied.The results indicated that the best carbon and nitrogen compounds for this strain were sucrose,peptone and ammonium nitrate respectively.The optimum producing condition was cultivate temperature at 30℃,pH of the fermentation medium at 5;medium quantity,10%;inoculation proportion,4%;rotate speed of the shaker,120r/min;fermentation time,96h.The highest enzyme activity could reach 2.63U/mL.

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Available abstract

The effect of the various carbon and nitrogen sources on fermentation of Penicillium P11 that produces phytase was studied.The results indicated that the best carbon and nitrogen compounds for this strain were sucrose,peptone and ammonium nitrate respectively.The optimum producing condition was cultivate temperature at 30℃,pH of the fermentation medium at 5;medium quantity,10%;inoculation proportion,4%;rotate speed of the shaker,120r/min;fermentation time,96h.The highest enzyme activity could reach 2.63U/mL.

Key concepts: Fermentation, Sucrose, Ammonium nitrate, Food science, Phytase, Penicillium, Chemistry, Inoculation

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