Effect of Different Concentrated Glucose on the p21 WAF1 Expression in Rat Peritoneal Mesothelial Cells

Objective To investigate the effect of different concentrated glucose on the p21WAF1 expression in rat peritoneal mesothelial cells. Methods Rat peritoneal mesothelial cells were cultured with serum free DMEM medium containing different glucose concentrations (13 8 g/L and 38 6 g/L) for 24 hours. The cell cycle distribution was then investigated by flow cytometry.. The expression of p21WAF1 was detected by RT PCR. Results In the presence of 13 8 g/L and 38 6 g/L glucose concentrations, most of the cells became hypertrophic, and were arrested in G 1 phase of cell cycle, which were obvious in the 38 6 g/L. High glucose stimulated expression of p21WAF1 mRNA, which was significantly higher in the 3 86%(P0 05). p21WAF1 mRNA expressions were absent in serum free normal glucose concentration groups and the D mannitol groups. Conclusion High glucose stimulated expression of p21WAF1, which might be related to the hypertrophy and arrest in the G 1 phase of mesothelial cells by high glucose.